Journal: Hypertension (Dallas, Tex. : 1979)

Article Title: Mild Hyperuricemia Attenuates Salt-Sensitive Hypertension and Kidney Damage

doi: 10.1161/HYPERTENSIONAHA.125.25550

Figure Lengend Snippet: Oxidative stress in the kidney. A , Differentially expressed (DE) genes known to affect the synthesis of reactive oxygen species (ROS) and oxidative stress in male rats. B , Western blot for AKR1B10 expression in the kidney and its quantification in male and female rats. C , Representative images of paraffin-embedded 8-OxodG-stained kidney and the quantification of the fluorescence intensity of the staining in male age-matched Sprague-Dawley (SD) rats fed a normal diet (healthy control), Dahl SS rats fed a high-salt (HS) diet, or Dahl SS rats fed a HS/oxonic acid diet. D , Representative images of optimal cutting temperature (OCT)-embedded 8-OxodG-stained kidney of the female Dahl SS rats fed a HS diet, or Dahl SS rats fed a HS/oxonic acid diet. Red-8OxodG, blue- nuclei. Values are shown as mean±SEM. An unpaired t test was used. P value **<0.01, ****<0.0001. Parts of the figure are created using BioRender.com.

Article Snippet: The membrane was first incubated with primary antibody for AKR1B10 (Cat# 18252-1-AP, Proteintech, Rosemont, IL) at 1:5000, then with a secondary antibody (Cat# 711-035-152, Peroxidase AffiniPure Donkey Anti-Rabbit IgG, Jackson ImmunoResearch Laboratories Inc., West Grove, PA) at 1:10000 in 2% BSA in Tris-buffered saline with Tween-20 (TBST).

Techniques: Western Blot, Expressing, Staining, Fluorescence, Control

Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Article Title: Overexpression and oncogenic function of aldo-keto reductase family 1B10 (AKR1B10) in pancreatic carcinoma

doi: 10.1038/modpathol.2011.191

Figure Lengend Snippet: Immunohistochemical analysis of AKR1B10 expression in human pancreatic tissues: ( A ) No detectable expression was observed in normal pancreatic acini or ducts. ( B ) Well-differentiated pancreatic ductal adenocarcinoma with high expression of AKR1B10, but negligible expression in an adjacent normal duct (open black arrow). ( C ) Precancerous pancreatic epithelial neoplasia also over-expressed AKR1B10 as demonstrated in pancreatic intraepithelial neoplasia-3 (large duct in center) and pancreatic intraepithelial neoplasia-1 lesions (open black arrows).

Article Snippet: AKR1B10 antibody (6μg/ml; Abnova Corporation, Taiwan) was applied overnight at 4°C followed by secondary staining using Vector Laboratories anti-mouse ImmPRESS Detection kit with bound antibody detected using Sigma FAST DAB for 5 minutes.

Techniques: Immunohistochemical staining, Expressing

Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Article Title: Overexpression and oncogenic function of aldo-keto reductase family 1B10 (AKR1B10) in pancreatic carcinoma

doi: 10.1038/modpathol.2011.191

Figure Lengend Snippet: Analysis of AKR1B10 expression along with corresponding enzyme activity. A) Using normal pancreatic ductal epithelial cell line (HPDE6E6E7) as a baseline control, and using the human hepatocellular carcinoma cell line (HepG2) as a positive control, western blot analysis revealed that AKR1B10 was over-expressed in all of six pancreatic adenocarcinoma cell lines, and that all of six cell lines exhibited either comparable or lower levels of expression to the HepG2 positive-control cell line. B) Oxidized NADPH was used as a monitor at 340 nm for measuring AKR1B10 enzymatic activity in these pancreatic adenocarcinoma cell lines, and showed that enzyme activity for all six cell lines paralleled AKR1B10 protein expression levels (A).

Article Snippet: AKR1B10 antibody (6μg/ml; Abnova Corporation, Taiwan) was applied overnight at 4°C followed by secondary staining using Vector Laboratories anti-mouse ImmPRESS Detection kit with bound antibody detected using Sigma FAST DAB for 5 minutes.

Techniques: Expressing, Activity Assay, Control, Positive Control, Western Blot

Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Article Title: Overexpression and oncogenic function of aldo-keto reductase family 1B10 (AKR1B10) in pancreatic carcinoma

doi: 10.1038/modpathol.2011.191

Figure Lengend Snippet: siRNA-mediated silencing of AKR1B10 expression and enzymatic activity. Western blot analysis demonstrates markedly decreased expression of AKR1B10 in knock-down cells (lower panel) with concomitant decrease in enzymatic activity (upper graph) that parallels AKR1B10 expression levels.

Article Snippet: AKR1B10 antibody (6μg/ml; Abnova Corporation, Taiwan) was applied overnight at 4°C followed by secondary staining using Vector Laboratories anti-mouse ImmPRESS Detection kit with bound antibody detected using Sigma FAST DAB for 5 minutes.

Techniques: Expressing, Activity Assay, Western Blot, Knockdown

Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Article Title: Overexpression and oncogenic function of aldo-keto reductase family 1B10 (AKR1B10) in pancreatic carcinoma

doi: 10.1038/modpathol.2011.191

Figure Lengend Snippet: AKR1B10 silencing resulted in induction of apoptosis and inhibition of cell proliferation. ( A ) Western blot analysis showed that increased level of cleaved caspase-8 protein, a marker of apoptosis, in Panc10.05 cells with AKR1B10 silencing. (B) Immunocytochemical analysis of cell apoptosis using activated caspase-3 in the scambled siRNA control ( B1 ) and those with siRNA silencing of AKR1B10 expression ( B2 ). Silencing of AKR1B10 expression resulted in increased activated caspase-3 labeled cell apoptosis as demonstrated by the arrows in the bottom image as well as the small size of the cell clones. The number of cells per clone was further counted and revealed that a significant decrease in the number of cells per clone were identified in Panc10.05 carcinoma cells with AKR1B10 silencing when compared to the parental control (18±9 vs. 32±11, p=0.048).

Article Snippet: AKR1B10 antibody (6μg/ml; Abnova Corporation, Taiwan) was applied overnight at 4°C followed by secondary staining using Vector Laboratories anti-mouse ImmPRESS Detection kit with bound antibody detected using Sigma FAST DAB for 5 minutes.

Techniques: Inhibition, Western Blot, Marker, Control, Expressing, Labeling, Clone Assay

Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Article Title: Overexpression and oncogenic function of aldo-keto reductase family 1B10 (AKR1B10) in pancreatic carcinoma

doi: 10.1038/modpathol.2011.191

Figure Lengend Snippet: Western blot analysis of prenylated proteins including HDJ2 and KRAS as well its downstream signals. Increased non-farnesylated HDJ2 protein ( upper black arrow ) and decreased membrane-bound KRAS protein were observed in the siRNA AKR1B10 pancreatic cancer cell line when compared to the scrambled siRNA control. Increased levels of membrane-bound E-cadherin ( lower black arrow ) and decreased levels of KRAS downstream effectors, phosphor-ERK and phosphor-MEK1/2, were also found. Flottin-2 was used as a loading control for membrane-bound proteins, while β-actin is used for cytosolic proteins.

Article Snippet: AKR1B10 antibody (6μg/ml; Abnova Corporation, Taiwan) was applied overnight at 4°C followed by secondary staining using Vector Laboratories anti-mouse ImmPRESS Detection kit with bound antibody detected using Sigma FAST DAB for 5 minutes.

Techniques: Western Blot, Membrane, Control

Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Article Title: Overexpression and oncogenic function of aldo-keto reductase family 1B10 (AKR1B10) in pancreatic carcinoma

doi: 10.1038/modpathol.2011.191

Figure Lengend Snippet: The proposed interactive role of AKR1B10 in carbonals, farnesyl and lipid metabolism as in maintaining the homeostasis of retinol to retinal that is involved in cellular apoptosis, protein prenylation and proliferation and contributes to pancreatic carcinogenesis.

Article Snippet: AKR1B10 antibody (6μg/ml; Abnova Corporation, Taiwan) was applied overnight at 4°C followed by secondary staining using Vector Laboratories anti-mouse ImmPRESS Detection kit with bound antibody detected using Sigma FAST DAB for 5 minutes.

Techniques:

Journal: Foods

Article Title: Auricularia auricula-judae Attenuates the Progression of Metabolic Syndrome in High-Fat Diet-Induced Obese Rats: Enzymatic Pre-Digestion Technology Is Superior to Superfine Grinding Method

doi: 10.3390/foods13030406

Figure Lengend Snippet: The primer sequences used in the real-time reverse transcription PCR analysis.

Article Snippet: The antibodies for Akr1b10 (E97823), Gpx1 (E91110), Srebfl (E2620152), Fasn (E90461), Cyp27a1 (E91982), Pnpla3 (E2516694), PPARg (E90270), and Lpin1(E98486) were purchased from EnoGene.

Techniques: Reverse Transcription

Journal: Liver International

Article Title: Up-regulated aldo-keto reductase family 1 member B10 in chronic hepatitis C: association with serum alpha-fetoprotein and hepatocellular carcinoma

doi: 10.1111/j.1478-3231.2012.02827.x

Figure Lengend Snippet: Differentially expressed genes in patients with elevated alpha-fetoprotein by fold change ranking (Top20)

Article Snippet: After blocking the endogenous peroxidase activity, the sections were incubated with a mouse monoclonal antibody against AKR1B10 (Ab 57547; Abcam, Cambridge, UK) with a 1:100 dilution at room temperature, followed by incubation with biotinylated secondary antibody (Ventana iVIEW DAB Universal Kit; Ventana Medical Systems Inc., Tucson, AZ, USA).

Techniques: Sequencing

Journal: Liver International

Article Title: Up-regulated aldo-keto reductase family 1 member B10 in chronic hepatitis C: association with serum alpha-fetoprotein and hepatocellular carcinoma

doi: 10.1111/j.1478-3231.2012.02827.x

Figure Lengend Snippet: Quantitative real-time RT-PCR analysis. (A) Comparison of AKR1B10 mRNA expression between the patients with normal alpha-fetoprotein (AFP) and the patients with elevated AFP (Mann–Whitney U -test, P < 0.001). (B) Regression of AKR1B10 mRNA and serum AFP ( N = 48, R 2 = 0.326, P < 0.001).

Article Snippet: After blocking the endogenous peroxidase activity, the sections were incubated with a mouse monoclonal antibody against AKR1B10 (Ab 57547; Abcam, Cambridge, UK) with a 1:100 dilution at room temperature, followed by incubation with biotinylated secondary antibody (Ventana iVIEW DAB Universal Kit; Ventana Medical Systems Inc., Tucson, AZ, USA).

Techniques: Quantitative RT-PCR, Comparison, Expressing, MANN-WHITNEY

Journal: Liver International

Article Title: Up-regulated aldo-keto reductase family 1 member B10 in chronic hepatitis C: association with serum alpha-fetoprotein and hepatocellular carcinoma

doi: 10.1111/j.1478-3231.2012.02827.x

Figure Lengend Snippet: AKR1B10 immnohistochemical analysis. (A) Representative immunohistochemical staining of normal control liver tissue. Bile duct epithelium served as the positive control. (B) Representative immunohistochemical staining of liver tissue with chronic hepatitis C (magnification × 100). (C) Quantification of AKR1B10 immunoreactivity (Mann–Whitney U -test). (D) Regression of AKR1B10 immunoreactivity and serum alpha-fetoprotein in patients with chronic hepatitis C ( N = 48, R 2 = 0.613, P < 0.001).

Article Snippet: After blocking the endogenous peroxidase activity, the sections were incubated with a mouse monoclonal antibody against AKR1B10 (Ab 57547; Abcam, Cambridge, UK) with a 1:100 dilution at room temperature, followed by incubation with biotinylated secondary antibody (Ventana iVIEW DAB Universal Kit; Ventana Medical Systems Inc., Tucson, AZ, USA).

Techniques: Immunohistochemical staining, Staining, Control, Positive Control, MANN-WHITNEY

Journal: Liver International

Article Title: Up-regulated aldo-keto reductase family 1 member B10 in chronic hepatitis C: association with serum alpha-fetoprotein and hepatocellular carcinoma

doi: 10.1111/j.1478-3231.2012.02827.x

Figure Lengend Snippet: Baseline characteristics of patients enrolled in the matched case-control study

Article Snippet: After blocking the endogenous peroxidase activity, the sections were incubated with a mouse monoclonal antibody against AKR1B10 (Ab 57547; Abcam, Cambridge, UK) with a 1:100 dilution at room temperature, followed by incubation with biotinylated secondary antibody (Ventana iVIEW DAB Universal Kit; Ventana Medical Systems Inc., Tucson, AZ, USA).

Techniques: Control, Expressing

Journal: Liver International

Article Title: Up-regulated aldo-keto reductase family 1 member B10 in chronic hepatitis C: association with serum alpha-fetoprotein and hepatocellular carcinoma

doi: 10.1111/j.1478-3231.2012.02827.x

Figure Lengend Snippet: Univariate and multivariate analysis for predictors of hepatocellular carcinoma development

Article Snippet: After blocking the endogenous peroxidase activity, the sections were incubated with a mouse monoclonal antibody against AKR1B10 (Ab 57547; Abcam, Cambridge, UK) with a 1:100 dilution at room temperature, followed by incubation with biotinylated secondary antibody (Ventana iVIEW DAB Universal Kit; Ventana Medical Systems Inc., Tucson, AZ, USA).

Techniques: Expressing

Journal: Liver International

Article Title: Up-regulated aldo-keto reductase family 1 member B10 in chronic hepatitis C: association with serum alpha-fetoprotein and hepatocellular carcinoma

doi: 10.1111/j.1478-3231.2012.02827.x

Figure Lengend Snippet: Cumulative incidence of hepatocellular carcinoma development according to AKR1B10 expression (log-rank test, P = 0.001).

Article Snippet: After blocking the endogenous peroxidase activity, the sections were incubated with a mouse monoclonal antibody against AKR1B10 (Ab 57547; Abcam, Cambridge, UK) with a 1:100 dilution at room temperature, followed by incubation with biotinylated secondary antibody (Ventana iVIEW DAB Universal Kit; Ventana Medical Systems Inc., Tucson, AZ, USA).

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: AKR1B10 inhibits the proliferation and migration of gastric cancer via regulating epithelial-mesenchymal transition

doi: 10.18632/aging.203538

Figure Lengend Snippet: Expression of AKR1B10 in gastric cancer tissues. ( A ) Representative immunohistochemistry images showing in situ AKR1B10 expression in gastric cancer (GC) and normal tissues (scale bar = 100 μm). ( B – D ) IHC scores of AKR1B10 in ( B ) GC vs normal tissues, ( C ) tumors with and without lymph node invasion, and ( D ) TNM stage I–II vs III–IV. ( E – G ) overall survival analysis of ( E ) AKR1B10 pos vs AKRiB10 neg GC patients, and in subgroups overall survival analysis of TNM stage I–II ( F ) and III–IV ( G ). LNM, Lymph node metastasis.

Article Snippet: Specific antibodies include: anti-AKR1B10 (1:1000; no. bs-6274R; Bioss), anti-E-cadherin (1:1000; no. bs-1016R; Bioss), anti-vimentin (1:1000; no. bs-23063R; Bioss) and anti-GAPDH (1:5000; no. bs-0755R; Bioss).

Techniques: Expressing, Immunohistochemistry, In Situ

Journal: Aging (Albany NY)

Article Title: AKR1B10 inhibits the proliferation and migration of gastric cancer via regulating epithelial-mesenchymal transition

doi: 10.18632/aging.203538

Figure Lengend Snippet: Relationship between AKR1B10 and clinic-pathological factors in GC patients.

Article Snippet: Specific antibodies include: anti-AKR1B10 (1:1000; no. bs-6274R; Bioss), anti-E-cadherin (1:1000; no. bs-1016R; Bioss), anti-vimentin (1:1000; no. bs-23063R; Bioss) and anti-GAPDH (1:5000; no. bs-0755R; Bioss).

Techniques:

Journal: Aging (Albany NY)

Article Title: AKR1B10 inhibits the proliferation and migration of gastric cancer via regulating epithelial-mesenchymal transition

doi: 10.18632/aging.203538

Figure Lengend Snippet: Results of univariate and multivariate analyses of postoperative patients’ survival by Cox’s proportional hazard model.

Article Snippet: Specific antibodies include: anti-AKR1B10 (1:1000; no. bs-6274R; Bioss), anti-E-cadherin (1:1000; no. bs-1016R; Bioss), anti-vimentin (1:1000; no. bs-23063R; Bioss) and anti-GAPDH (1:5000; no. bs-0755R; Bioss).

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: AKR1B10 inhibits the proliferation and migration of gastric cancer via regulating epithelial-mesenchymal transition

doi: 10.18632/aging.203538

Figure Lengend Snippet: Subgroup analysis of the influence of AKR1B10 expression on the survival of gastric cancer patients.

Article Snippet: Specific antibodies include: anti-AKR1B10 (1:1000; no. bs-6274R; Bioss), anti-E-cadherin (1:1000; no. bs-1016R; Bioss), anti-vimentin (1:1000; no. bs-23063R; Bioss) and anti-GAPDH (1:5000; no. bs-0755R; Bioss).

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: AKR1B10 inhibits the proliferation and migration of gastric cancer via regulating epithelial-mesenchymal transition

doi: 10.18632/aging.203538

Figure Lengend Snippet: Effect of AKR1B10 on the cell proliferation and migration ability of gastric cancer cells. ( A ) AKR1B10 expression in gastric cancer (GC) cell lines from the CCLE platform. ( B ) Relative expression of AKR1B10 in GC cell lines according to RNAseq results via the CCLE platform. ( C – D ) Immunoblot showing AKR1B10 protein levels in MKN45 cells transfected with AKR1B10-shRNA ( C ) and in AGS cells transfected with the AKR1B10 overexpression plasmid ( D ), and gray value analysis via ImageJ. ( E – J ) Proliferation rates ( E – F ), colony forming ability ( G – H ), and migration ability ( I – J ) of AKR1B10-KD and AKR1B10-OE GC cells. CCLE, Cancer Cell Line Encyclopedia. NC, negative control. KD, knockdown, AKR1B10-shRNA. VEC, vector. OE, overexpression, AKR1B10 overexpression plasmid. Data are presented as the mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Specific antibodies include: anti-AKR1B10 (1:1000; no. bs-6274R; Bioss), anti-E-cadherin (1:1000; no. bs-1016R; Bioss), anti-vimentin (1:1000; no. bs-23063R; Bioss) and anti-GAPDH (1:5000; no. bs-0755R; Bioss).

Techniques: Migration, Expressing, Western Blot, Transfection, shRNA, Over Expression, Plasmid Preparation, Negative Control

Journal: Aging (Albany NY)

Article Title: AKR1B10 inhibits the proliferation and migration of gastric cancer via regulating epithelial-mesenchymal transition

doi: 10.18632/aging.203538

Figure Lengend Snippet: Correlation between AKR1B10 and epithelial-mesenchymal transition. ( A ) Correlation analysis of AKR1B10 and Vimentin gene expression levels in gastric cancer (GC) patients by the GEPIA platform. ( B ) Correlation analysis of AKR1B10 and E-cadherin gene expression levels in GC patients by the GEPIA platform. ( C – E ) Comparison of AKR1B10 ( C ), Vimentin ( D ) and E-cadherin ( E ) mRNA levels in 19 paired GC and normal tissues. ( F ) Correlation between AKR1B10 and Vimentin, and between AKR1B10 and E-cadherin mRNA levels in GC tissues. ( G ) MKN45 cells transfected with NC or KD and ( H ) AGS transfected with VEC or AKR1B10-OE. The bands were semi-quantified by ImageJ and the results are presented as the mean ± SD. VIM, Vimentin; ECAD, E-cadherin; TPM, transcripts per million. NC, negative control; KD, knockdown; VEC, vector; OE, overexpression. ** P < 0.01, *** P < 0.001.

Article Snippet: Specific antibodies include: anti-AKR1B10 (1:1000; no. bs-6274R; Bioss), anti-E-cadherin (1:1000; no. bs-1016R; Bioss), anti-vimentin (1:1000; no. bs-23063R; Bioss) and anti-GAPDH (1:5000; no. bs-0755R; Bioss).

Techniques: Expressing, Transfection, Negative Control, Plasmid Preparation, Over Expression

Journal: Aging (Albany NY)

Article Title: AKR1B10 inhibits the proliferation and migration of gastric cancer via regulating epithelial-mesenchymal transition

doi: 10.18632/aging.203538

Figure Lengend Snippet: AKR1B10 knockdown promotes gastric cancer tumor growth in vivo . ( A – B ) Total body weight ( A ) and tumor volume ( B ) of the mice. ( C ) Representative pictures of subcutaneous tumors harvested from NC and AKR1B10-KD group. ( D ) The weights of tumor masses. ( E ) The weights of mice without tumor masses. ( F – I ) Relative AKR1B10 ( F ), Vimentin ( G ) and E-cadherin ( H ) mRNA levels in tumors of the AKR1B10-KD or NC group, and their correlation ( I ). ( J ) Stratification of mice into cluster 1 and cluster 2 according to AKR1B10 mRNA levels, body weight and tumor weight. ( K ) Percentage of NC and AKR1B10-KD mice in each cluster. Data are presented as the mean ± SD. NC, negative control; KD, AKR1B10 knockdown. * P < 0.05, ** P < 0.01.

Article Snippet: Specific antibodies include: anti-AKR1B10 (1:1000; no. bs-6274R; Bioss), anti-E-cadherin (1:1000; no. bs-1016R; Bioss), anti-vimentin (1:1000; no. bs-23063R; Bioss) and anti-GAPDH (1:5000; no. bs-0755R; Bioss).

Techniques: In Vivo, Negative Control